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BIOTAGE psq96 hs platform
Psq96 Hs Platform, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psq96+hs+platform/psq+96+snp+reagent+kit/pmc06497304-88-43-46
Average 90 stars, based on 1 article reviews
psq96 hs platform - by Bioz Stars, 2026-09
90/100 stars

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Sequencing:

Article Title: Analysis of mutational dynamics at the DMPK (CTG) n locus identifies saliva as a suitable DNA sample source for genetic analysis in myotonic dystrophy type 1
Article Snippet: Isolated products were dispensed into optical plates containing 12 μl of the corresponding sequencing primer (either PS-DMPK-S3 for CTCF1, or PS-DMPK-S4 for CTCF2) dissolved in annealing buffer (Biotage, USA) to a final concentration of 0.4 μM. .. To allow annealing of the sequencing primer to the template, plates were incubated for 5 min in a heating block at 85°C, left to cool for 5 min and then placed at room temperature for 5 min. Pyrosequencing was carried out using the PSQ96 HS platform (Biotage, USA) and PyroMark Gold Q96 reagents (Biotage, USA) according to the manufacturer’s instructions and analysed with Q-CpG software (Biotage, USA), which estimates the methylation percentage for each of the interrogated CpG sites. ..

Incubation:

Article Title: Analysis of mutational dynamics at the DMPK (CTG) n locus identifies saliva as a suitable DNA sample source for genetic analysis in myotonic dystrophy type 1
Article Snippet: Isolated products were dispensed into optical plates containing 12 μl of the corresponding sequencing primer (either PS-DMPK-S3 for CTCF1, or PS-DMPK-S4 for CTCF2) dissolved in annealing buffer (Biotage, USA) to a final concentration of 0.4 μM. .. To allow annealing of the sequencing primer to the template, plates were incubated for 5 min in a heating block at 85°C, left to cool for 5 min and then placed at room temperature for 5 min. Pyrosequencing was carried out using the PSQ96 HS platform (Biotage, USA) and PyroMark Gold Q96 reagents (Biotage, USA) according to the manufacturer’s instructions and analysed with Q-CpG software (Biotage, USA), which estimates the methylation percentage for each of the interrogated CpG sites. ..

Blocking Assay:

Article Title: Analysis of mutational dynamics at the DMPK (CTG) n locus identifies saliva as a suitable DNA sample source for genetic analysis in myotonic dystrophy type 1
Article Snippet: Isolated products were dispensed into optical plates containing 12 μl of the corresponding sequencing primer (either PS-DMPK-S3 for CTCF1, or PS-DMPK-S4 for CTCF2) dissolved in annealing buffer (Biotage, USA) to a final concentration of 0.4 μM. .. To allow annealing of the sequencing primer to the template, plates were incubated for 5 min in a heating block at 85°C, left to cool for 5 min and then placed at room temperature for 5 min. Pyrosequencing was carried out using the PSQ96 HS platform (Biotage, USA) and PyroMark Gold Q96 reagents (Biotage, USA) according to the manufacturer’s instructions and analysed with Q-CpG software (Biotage, USA), which estimates the methylation percentage for each of the interrogated CpG sites. ..

Software:

Article Title: Analysis of mutational dynamics at the DMPK (CTG) n locus identifies saliva as a suitable DNA sample source for genetic analysis in myotonic dystrophy type 1
Article Snippet: Isolated products were dispensed into optical plates containing 12 μl of the corresponding sequencing primer (either PS-DMPK-S3 for CTCF1, or PS-DMPK-S4 for CTCF2) dissolved in annealing buffer (Biotage, USA) to a final concentration of 0.4 μM. .. To allow annealing of the sequencing primer to the template, plates were incubated for 5 min in a heating block at 85°C, left to cool for 5 min and then placed at room temperature for 5 min. Pyrosequencing was carried out using the PSQ96 HS platform (Biotage, USA) and PyroMark Gold Q96 reagents (Biotage, USA) according to the manufacturer’s instructions and analysed with Q-CpG software (Biotage, USA), which estimates the methylation percentage for each of the interrogated CpG sites. ..

Methylation:

Article Title: Analysis of mutational dynamics at the DMPK (CTG) n locus identifies saliva as a suitable DNA sample source for genetic analysis in myotonic dystrophy type 1
Article Snippet: Isolated products were dispensed into optical plates containing 12 μl of the corresponding sequencing primer (either PS-DMPK-S3 for CTCF1, or PS-DMPK-S4 for CTCF2) dissolved in annealing buffer (Biotage, USA) to a final concentration of 0.4 μM. .. To allow annealing of the sequencing primer to the template, plates were incubated for 5 min in a heating block at 85°C, left to cool for 5 min and then placed at room temperature for 5 min. Pyrosequencing was carried out using the PSQ96 HS platform (Biotage, USA) and PyroMark Gold Q96 reagents (Biotage, USA) according to the manufacturer’s instructions and analysed with Q-CpG software (Biotage, USA), which estimates the methylation percentage for each of the interrogated CpG sites. ..



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BIOTAGE psq96 hs platform
Psq96 Hs Platform, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psq96+hs+platform/psq+96+snp+reagent+kit/pmc06497304-88-43-46
Average 90 stars, based on 1 article reviews
psq96 hs platform - by Bioz Stars, 2026-09
90/100 stars
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